Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid (gas, solvent, water) called the mobile phase, which carries it through a system (a column, a capillary tube, a plate, or a sheet) on which is fixed a material called the stationary phase.
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC):
It is a form of column chromatography that pumps a sample mixture or analyte in a solvent (known as the mobile phase) at high pressure through a column with chromatographic packing material (stationary phase). The sample is carried by a moving carrier gas stream of helium or nitrogen.
Depending on the chemical structure of the sample, the molecules are slowed down while passing through the stationary phase. The specific interactions between the molecules of a sample and the packing material define their time. Hence, different constituents of a sample are eluted at different times. Thereby, the separation of the sample ingredients is achieved.
WHAT ARE CHROMATOGRAPHY FRITS?
Chromatography Frits are Inert physical filtration devices, typically installed before pre-columns, that provide a coarse filtration of the HPLC eluent and prevent contaminant particles from reaching the HPLC system.
Frits are used to eliminate the traditional inline filters and reduce the number of connections in the process
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APPLICATION OF HPLC FRITS:
Manufacturing: HPLC frits have many applications in both laboratory and clinical science. It is a common technique used in pharmaceutical development, as it is a dependable way to obtain and ensure product purity. While HPLC can produce extremely high quality (pure) products, it is not always the primary method used in the production of bulk drug materials.
Research: Similar assays can be performed for research purposes, detecting concentrations of potential clinical candidates like antifungal and asthma drugs. This technique is obviously useful in observing multiple species in collected samples, as well, but requires the use of standard solutions.
Medical: Medical use of HPLC can include drug analysis, but falls more closely under the category of nutrient analysis. While urine is the most common medium for analyzing drug concentrations, blood serum is the sample collected for most medical analyses with HPLC
Some other applications:
- Water purification
- Detection of impurities in pharmaceutical industries
- Pre-concentration of trace components
- Ligand-exchange chromatography
- Ion-exchange chromatography of proteins
- High-pH anion-exchange chromatography of carbohydrates and oligosaccharides